TY - JOUR AU - Omagari, Katsuhisa AU - Suruga, Kazuhito AU - Kyogoku, Akira AU - Nakamura, Satomi AU - Sakamoto, Ai AU - Nishioka, Shinta AU - Ichimura, Mayuko AU - Miyata, Yuji AU - Tajima, Koichi AU - Tsuneyama, Koichi AU - Tanaka, Kazunari PY - 2017 TI - A fermented mixed tea made with camellia (Camellia japonica) and third-crop green tea leaves prevents nonalcoholic steatohepatitis in Sprague-Dawley rats fed a high-fat and high-cholesterol diet JF - Hepatobiliary Surgery and Nutrition; Vol 7, No 3 (June 21, 2018): Hepatobiliary Surgery and Nutrition Y2 - 2017 KW - N2 - Background: Established treatments for non-alcoholic steatohepatitis (NASH) are few, thus it is imperative to develop novel dietary strategies that can prevent NASH. A fermented mixed tea (FMT) made with Camellia japonica (Japanese camellia) and third- crop green tea leaves by tea-rolling processing was reported to reduce body weight and adipose tissue weight in Sprague-Dawley (SD) rats. Because visceral fat is one of the most important factors for the development of hepatic steatosis, this FMT supplementation can be a candidate dietary strategy for the prevention of NASH. Methods: Nine-week-old male SD rats were fed a high-fat and high-cholesterol (HFC) diets with or without FMT (camellia and third-crop green tea leaves at ratios of 1:5, 1:2 and 1:1) for 9 weeks (n=6–7/ group). Histopathology, serology and expressions of fibrogenetic, proinflammatory, oxidative stress and lipid metabolism-related genes in the liver were evaluated. Results: Histologically, HFC diet with FMT at a ratio of 1:5 dramatically reduced NASH progression (14%) compared to the HFC diet without FMT (100%). FMT at a ratio of 1:5 reduced hepatic steatosis due to the activation of microsomal triglyceride transfer protein, and FMT at a ratio of 1:2 reduced mRNA levels of some proinflammatory, lipid metabolism-related, fibrogenic and oxidative stress marker genes. Conclusions: Our data suggest that FMT at a ratio of 1:5 or 1:2 likely possesses a preventive effect on NASH progression. UR - https://hbsn.amegroups.org/article/view/16564